Is Annatto (E160b) Vegan?

Also known as: Bixin, Norbixin, Annatto extract, Achiote

Vegan

Extracted from the seeds of the Bixa orellana (achiote) tree, native to tropical regions of Central and South America. Entirely plant-derived.

Ingredient Data

Vegan Status

Vegan

E-Number

E160b

Also Known As

Bixin; Norbixin; Annatto extract; Achiote

Source

Extracted from the seeds of the Bixa orellana (achiote) tree, native to tropical regions of Central and South America. Entirely plant-derived.

Commonly Found In

Cheese (particularly Red Leicester, Double Gloucester, Edam, Gouda), butter, margarine, smoked fish, rice dishes, snack foods.

Vegan Alternative

No substitute needed — E160b is vegan. Ironically, annatto is often used in non-vegan cheeses but is itself always plant-based.

Additional Notes

One of the oldest natural food colorants. Used by indigenous communities in the Americas for centuries as a body paint and food dye. The primary colourant in many commercially produced cheeses — the orange colour of Red Leicester and American cheddar is annatto, not natural cheese colour. When you see an orange cheese, the annatto is vegan even if the cheese is not.

In depth

How Annatto Is Produced

Annatto is obtained from the seeds of Bixa orellana, a small tropical tree sometimes called the achiote or lipstick tree. The tree is native to tropical Central and South America and is now also cultivated in parts of Africa and Asia. The pigment is not inside the seed itself but in the waxy reddish coating that surrounds it.

Processing separates this coating from the seeds in one of two main ways. Extraction with vegetable oil or food-grade solvents yields bixin, the fat-soluble form of the pigment, which suits fatty foods such as cheese, butter, and margarine. Treatment with an alkaline water solution converts the pigment to norbixin, a water-soluble form used in beverages, snack coatings, and other water-based applications.

Every stage of production is plant-based. The raw material is a seed coating, the extraction media are oils, solvents, or alkaline water, and no animal-derived processing aids are standard in annatto manufacture. The finished colorant ranges from yellow to orange-red depending on concentration and the form used.

How to Spot It on Labels

In the European Union, annatto appears on ingredient lists as E160b or by name, and regulatory listings distinguish between the bixin and norbixin forms. In the United States, it is classified among color additives exempt from certification and is typically declared as "annatto," "annatto extract," or "annatto (color)."

Other names can signal its presence. "Bixin" and "norbixin" refer to the two pigment forms. On Latin American products, "achiote" or "achiote paste" indicates annatto, often combined with spices. Filipino products may list "atsuete," and French Caribbean products sometimes use "roucou." All of these refer to the same plant-derived colorant.

Because annatto is a natural color, some products describe it in marketing terms such as "colored with natural annatto" rather than listing an E-number. Whatever the phrasing, the ingredient itself is always vegan. The relevant label question for vegans is not the annatto but the food around it — the cheese, butter, or smoked fish it colors is frequently animal-derived.

Why So Much Cheese Is Orange

Cheese made from cow's milk is naturally white to pale yellow, with the depth of color varying by season and diet. Historically, milk from cows grazing on fresh summer pasture contained more carotene and produced a yellower cheese that was associated with higher quality. Cheesemakers began adding annatto to reproduce that color year-round, and the practice became standard for several traditional styles.

Red Leicester and Double Gloucester in England, Mimolette in France, and much of the cheddar sold in the United States owe their orange color to annatto rather than to the milk itself. The same logic applies to butter and margarine, where annatto helps deliver a consistent yellow regardless of season.

For vegans, this creates a recurring point of confusion: annatto is one of the few ingredients in a conventional cheese that is reliably plant-based. Seeing E160b on a cheese label says nothing about the product being vegan — the milk, cultures, and often the rennet remain animal-derived. Vegan cheese producers also use annatto to achieve cheddar-style coloring.

Culinary Uses Beyond Coloring

Outside industrial food coloring, annatto is a cooking ingredient in its own right. In Mexican and Central American cuisine, ground achiote seeds are blended with spices and sour citrus into achiote paste, the base of Yucatecan dishes such as cochinita pibil. In Puerto Rican and other Caribbean cooking, seeds are steeped in hot oil to make an orange-tinted cooking fat, and achiote is a common component of sazón seasoning blends. Filipino cuisine uses the seeds, known as atsuete, to color stews and sauces.

At these culinary concentrations, annatto contributes a mild flavor often described as earthy, peppery, or slightly nutty. At the much lower levels used as a commercial colorant, it is essentially flavorless, which is part of why it is so widely used.

The seeds and pastes sold for home cooking are plain plant products, though commercial achiote pastes should still be checked for added ingredients. The plain seeds, powder, and oil preparations are vegan by default.

Frequently asked questions

Is annatto vegan?

Yes, annatto is always vegan. It is extracted from the seed coating of the Bixa orellana tree, a purely plant-based source, and no animal-derived materials are standard in its production. The foods it colors, such as cheese and butter, are often not vegan, but the colorant itself is.

Is E160b vegan?

Yes. E160b is the European food additive number for annatto, including its bixin and norbixin forms, and all of them come from the seeds of a tropical tree. There is no animal-derived version of E160b.

Why is cheddar cheese orange?

Most orange cheddar gets its color from added annatto, not from the milk. Cheesemakers originally used it to imitate the yellower cheese produced from carotene-rich summer milk, and the practice became standard for styles like American cheddar, Red Leicester, and Double Gloucester. The annatto is vegan, but the cheese itself usually is not.

Is annatto the same as achiote?

Yes, annatto and achiote refer to the same plant, Bixa orellana, and the colorant made from its seeds. "Achiote" is the common name in Spanish-speaking countries and usually refers to the whole seeds or spice pastes used in cooking, while "annatto" is the usual name for the extracted food colorant.

Is annatto natural or artificial?

Annatto is a natural colorant. It is extracted directly from plant seeds rather than synthesized, and in the United States it is regulated among color additives exempt from certification, a category that mainly covers colors from natural sources. It is one of the oldest food dyes in continuous use.

Does annatto have a taste?

At the low levels used as a food colorant, annatto is essentially flavorless. In larger culinary amounts, such as in achiote paste or annatto-infused oil, it has a mild earthy, slightly peppery, nutty flavor. Its role in most packaged foods is purely visual.

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